000			04995cam a22004217a 4500
003			BUC
005			20220405043358.0
008			200121s2011 nyua g b i 001 0 eng
010			_a2010937423
020			_a9781603271868 (alk. paper)
020			_a1603271864 (alk. paper)
020			_a9781607618492 (eISBN)
020			_a1607618494 (eISBN)
040			_aBADR UNIVERSITY IN CAIRO _cBADR UNIVERSITY IN CAIRO _beng _erda
041	0		_aeng
050	0	0	_aRM301.25 _b.D7864 2011
082	0	0	_222 _a616.107 _bDRS
245	0	0	_aDrug safety evaluation : _bmethods and protocols / _cedited by Jean-Charles Gautier.
260			_aNew York, NY : _bHumana Press, _cc2011.
300			_axiii, 431 p. : _bill ; _c26 cm.
336			_236
490	1		_aMethods in molecular biology, _v691
490	1		_aSpinger protocols
504			_aIncludes bibliographical references and index.
505			_aPart I. General toxicology. Developing combination drugs in preclinical studies / Alberto Lodola. Preclinical evaluation of juvenile toxicity / Paul C. Barrow, Stéphane Barbellion, and Jeanne Stadler -- Part II. Pathology. Necropsy and sampling procedures in rodents / Laurence Fiette and Mohamed Slaoui. Histopathology procedures : from tissue sampling to histopathological evaluation / Mohamed Slaoui and Laurence Fiette. Principles and methods of immunohistochemistry / José A. Ramos-Vara. Tissue microarrays and digital image analysis / Denise Ryan, Laoighse Mulrane, Elton Rexhepaj, and William M. Gallagher -- Part III. Genetic toxicology. Micronucleus assay and labeling of centromeres with FISH technique / Ilse Decordier, Raluca Mateuca, and Micheline Kirsch-Volders. The use of bacterial repair endonucleases in the comet assay / Andrew R. Collins -- Part IV. Safety pharmacology. Manual whole-cell patch-clamping of the HERG cardiac K⁺ channel / Xiao-Liang Chen, Jiesheng Kang, and David Rampe -- Part V. Investigative toxicology. Generation and analysis of transcriptomics data / Philip D. Glaves and Jonathan D. Tugwood. Protocols of two-dimensional difference gel electrophoresis to investigate mechanisms of toxicity / Emmanuelle Com, Albrecht Gruhler, Martine Courcol, and Jean-Charles Gautier. Protocols and applications of cellular metabolomics in safety studies using precision-cut tissue slices and carbon 13 NMR / Gabriel Baverel, Sophie Renault, Hassan Faiz, Maha El Hage, Catherine Gauthier, Agnès Duplany, Bernard Ferrier, and Guy Martin. Statistical analysis of quantitative RT-PCR results / Richard Khan-Malek and Ying Wang. Evaluation of mitochondrial respiration in cultured rat hepatocytes / Jean-Pierre Marchandeau and Gilles Labbe -- Part VI. Screening assays for developmental toxicity. FETAX assay for evaluation of developmental toxicity / Isabelle Mouche, Laure Malesic, and Olivier Gillardeaux. Evaluation of embryotoxicity using the zebrafish model / Lisa Truong, Stacey L. Harper, and Robert L. Tanguay -- Part VII. Chemical protein adducts. Protocols of in vitro protein covalent binding studies in liver / Jean-François Lévesque, Stephen H. Day, and Allen N. Jones. Utilization of MALDI-TOF to determine chemical-protein adduct formation in vitro / Ashley A. Fisher, Matthew T. Labenski, Terrence J. Monks, and Serrine S. Lau. Utilization of LC-MS/MS analyses to identify site-specific chemical protein adducts in vitro / Ashley A. Fisher, Matthew T. Labenski, Terrence J. Monks, and Serrine S. Lau. One-dimensional western blotting coupled to LC-MS/MS analysis to identify chemical-adducted proteins in rat urine / Matthew T. Labenski, Ashley A. Fisher, Terrence J. Monks, and Serrine S. Lau. Identification of chemical-adducted proteins in urine by multi-dimensional protein identification technology (LC/LC-MS/MS) / Matthew T. Labenski, Ashley A. Fisher, Terrence J. Monks, and Serrine S. Lau -- Part VIII. Safety biomarkers. Optimization of SELDI for biomarker detection in plasma / Jean-Francois Léonard, Martine Courcol, and Jean-Charles Gautier. Differential proteomics incorporating iTRAQ labeling and multi-dimensional separations / Ben C. Collins, Thomas Y.K. Lau, Stephen R. Pennington, and William M. Gallagher. NMR and MS methods for metabonomics / Frank Dieterle, Björn Riefke, Götz Schlotterbeck, Alfred Ross, Hans Senn, and Alexander Amberg. Absolute quantification of toxicological biomarkers by multiple reaction monitoring / Thomas Y. K. Lau, Ben C. Collins, .Peter Stone, Ning Tang, William M. Gallagher, and Stephen R. Pennington
650		0	_aDrugs _xToxicology.
650		0	_aDrugs _xTesting.
650	1	2	_aDrug Evaluation, Preclinical _xmethods.
650	2	2	_aDrug Toxicity.
650	2	2	_aToxicity Tests _xmethods.
700	1		_a Gautier, Jean-Charles.
830		0	_aMethods in molecular biology (Clifton, N.J.) ; _vv. 691.
830		0	_aSpringer protocols.
856	4	1	_uhttp://elib2.cdc.gov:3273/BookToc/doi/10.1007/978-1-60761-849-2
902			_aE.Anwer
942			_2ddc _cBK
999			_c2228 _d2228